Partner 5

 Dr Danièle Werck-Reichhart, PI

IBMP is the largest CNRS facility dedicated to plant sciences and is considered as a leading European Centre of Excellence in the field. It is formed of four departments respectively dedicated to Integrative virology, Regulation and coordination of Genome expression, Molecular Mechanisms of phenotypic plasticity and Plant metabolic Networks. IBMP has implemented several technical platforms, including Plant production, Bio-imaging, Bioinformatics and structural biology, Microscopy, Protein production and proteomics, DNA-sequencing and qPCR, and Metabolomics and analysis of small molecules. The latter is so far equipped with UPLC-MS/MS, GC-FID, GC-MS, GC-MS with thermal desorption and a system for the collection of volatile compounds. IBMP generates yearly €10M in research funding, supporting a group of ca. 180 scientists, including 19 principal investigators.

IPBMP - CNRS

Danièle Werck-Reichhart is Research Director at CNRS and Head of the Plant Cytochromes P450 Group and of the Department Plant Metabolic Networks of IBMP. She completed her PhD at University of Strasbourg under the supervision of Francis Durst. She has been working as a postdoc for agrochemical industry on pesticide metabolism, and at University of Bristol on plant heme biosynthesis. She was trained in plant genomics at the Carnegie Institute of Stanford in the group of Chris Somerville. She has been working with P450 enzymes since the 1980s and her present interest is in the identification of new plant P450 functions based on genome analysis and gene expression data. Work is presently focused on flower development and the biosynthesis of biopolymers, bioactive molecules, aroma and scents. She has published more than 100 papers in the field, including many reviews, and coordinated national and international research projects.

P4FIFTY project in the IBMP

Characterization of P450s responsible of grape aromas The diversity of grapevine secondary metabolism is the source of an exceptional diversity of compounds that contribute greatly to the aromatic and color complexity of wine. The analysis of the grapevine genome has shown a remarkable expansion of several gene families linked to secondary metabolism, compared to other plants. A striking example is the blooming of some P450 subfamilies, in particular those recently shown to be involved in flavonoids and of terpene oxidation in the CNRS lab. Taking advantage of the forthcoming availability of several wild and cultivated Vitis genome sequences, the aim of the project is to assess the impact of this expansion on the production and diversification of grape berry pigments and aroma.

Staff undertaking the work

Nicolas Navrot, assistant professor with some logistic support of: Agnes Lesot, assistant engineer Raphael Lugan, engineer of the metabolomic platform Our partners of INRA Colmar for grapevine genetics, genomics and sequencing